Word: coli
(lookup in dictionary)
(lookup stats)
Dates: during 1970-1979
Sort By: most recent first
(reverse)
...past year, the Holy Grail of genetic engineering has been the production of human insulin by E. coli bacteria cells. This work, if successful, would provide an almost unlimited source of natural human insulin for diabetics at a low price, a very tangible medical benefit which would go far towards convincing the public of the benefits of recombinant DNA research...
...California groups, led by Howard Goodman and Bill Ruggers, inserted the insulin gene already in bacteria last year but they have been unsuccessful in getting the E. coli to read it, according to Gilbert. The other West Coast project, run by Genentech Inc. and an organic chemist, Dr. Keiichi Itakura, announced in September that it had successfully produced human insulin using E. coli bacteria...
Itakura and his associates reported that, "Artificial genes that 'command' laboratory bacteria to manufacture human insulin have been synthesized." Rather than using natural animal genes for insulin, this group built an artificial copy of the human insulin gene in two short segments and inserted these separately into E. coli plasmids...
...just a fantastic and powerful tool," Doty says. This method of including E. coli to clone many copies of the DNA template is cheap, efficient, and, above all, it produces pure mixtures of the DNA. Despite gene splicing abilities, which may speed up the work by years, Doty says without any hint of discouragement, "disecting out what any of this means is going to take a tremendous amount of time...
...interferon molecule and thus cannot bring down the cost by synthesizing it. Nor have they isolated the gene that orders interferon production in the cell. Once that gene is determined, Gutterman says, the technique of recombinant DNA could be used to insert it into a laboratory strain of E. coli bacteria, which would then multiply and produce interferon inexpensively and in large quantities...