Word: frozenly
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...meantime, Eggan's group has provided an alternative method for generating customized stem cells that would take advantage of the early-stage embryos frozen in IVF centers around the country. The most reliable way of generating patient-specific stem cells remains nuclear transfer-taking the nucleus from a patient's skin cell and inserting it into an egg that has had its nucleus removed. This hybrid then begins to divide, and within a few days, generates stem cells that are genetically identical to the patient. The problem, however, as Eggan puts it, is that "there are never any extra unfertilized...
...plenty to be proud of and plenty of positives to reflect on following an exciting and hard-fought season.After losing its three stars to the Winter Olympics in Turin the previous year and proving it could play without them by winning the ECAC Tournament and making an NCAA Frozen Eight appearance, Harvard had a lot to look forward to at the beginning of the season with the return of co-captain Julie Chu, junior Caitlin Cahow, and sophomore Sarah Vaillancourt.The trio made an immediate impact, helping the Crimson rattle off four straight wins out of the gate and adding...
...Somewhere between 400,000 and 500,000 such embryos lie in frozen animation in IVF clinics across the U.S. Couples have the option of keeping these embryos frozen, discarding them, donating them to other infertile couples, or making them available for study. Because President Bush has forbidden federal funds to be used to study human embryonic stem cells, only privately supported programs can ask couples with excess embryos to donate them for stem cell research. Given the high cost of each IVF cycle, however, more and more couples expressed a desire to keep their stem cells from themselves...
...Stem cell lines are not easy to generate, however, so StemLifeLine requests that interested couples donate at least 10 embryos if they want to take advantage of the service. Those frozen embryos will be shipped from the IVF center to the company's San Francisco facility at a cost of several thousand dollars (Krtolica declined to provide an exact figure, citing ongoing talks with a few IVF clinics). There scientists thaw and nurture the embryos to generate stem cells. These are then removed, cultured and allowed to grow. The surviving colonies are then frozen and kept cryopreserved in liquid nitrogen...
...Krtolica maintains that the frozen embryos StemLifeLine starts out with are only slightly less viable than fresh ones in generating stem cells lines, and that freezing cells is a routine and well understood process. So far, she says, there is no evidence that frozen stem cell lines are any less active or viable when thawed - but scientists don't yet have a lot of experience in manipulating and storing human stem cells. The most harmful part of the process, says Krtolica, is the freezing itself; "the freezing process has the biggest harm in killing cells," she says. "The number...