Word: stems
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...Despite the retraction, Hwang insists he will provide proof of his work in 10 days. Hwang maintains that he did create the groundbreaking stem cell lines from patients, and that he even provided them at the time to six researchers who can vouch for their authenticity. "What I can say for sure is that we did create patient-specific stem cells and we have source technologies to repeat them from scratch," he said, referring to the stem cells he cultured for the first time from 11 patients with diseases...
...Hwang did not specifically address the most serious charge which aired on the Korean network MBC-TV on Thursday night. In an interview, one of his junior researchers claimed that Hwang had asked him to turn two or three stem cell lines into 11 by photographing them repeatedly for submission to Science...
...Instead, Hwang acknowledged that the images published in Science were problematic, but provided his own explanation: that the stem cells were somehow mismanaged or possibly switched. In January, his team at Seoul National University had created six patient-specific stem cell lines, but they became contaminated. They reported the breach to the government and rescued two batches which they stored temporarily at their partner lab in Mizmedi Hospital. After Hwang's lab was cleaned and new sterilization procedures put into place, the stem cell lines came back to Hwang's lab. His team then went on to create six more...
...When producers of an investigative television program from MBC-TV raised questions about the validity of the stem cells following the egg-donor scandal, Hwang provided MBC-TV with samples from five stem cell lines, and cells from their donors, in an effort to prove their authenticity. It's not clear where these stem cells were stored. Hwang said he and his colleagues performed their own in-house test, including DNA fingerprinting to verify the source of the stem cells. It was then that he discovered that the fingerprints did not match those printed in Science. "We learned that...
...team is thawing five frozen stem cell lines and conducting DNA analysis to verify that he was indeed successful in using cloning techniques to extract stem cells from patients with diseases; those results will be available in 10 days. After that, Hwang will have either answered his critics or he'll have even more questions to face...