Word: sueoka
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Dates: during 1960-1960
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...University scientist has found that a one-celled green plant, an alga, duplicates its transmitters of genetic information (DNA) in the same way as bacteria. This discovery of Noboru Sueoka, research fellow in Biology, reinforces the theory that at the fundamental level of biochemistry, most living processes are the same...
...deoxyribonucleic acid) occurs in all living cells as long chains of molecules. In reproduction it passes on a "blueprint" governing cellular chemical events. Sueoka has shown that when a cell of the green alga, Chlamydomonas reinhardi, divides into two daughter cells, the new cells each contain half of the original DNA molecule and also a newly synthesized portion...
...long strands of atoms are coiled together to make a spiral. Pairs of molecules called "nitrogen bases" connect the coils, which unwind in the process of duplication and assemble themselves on new strands. A series of experiments done a year ago at the California Institute of Technology, after which Sueoka patterned his experiments, supports this hypothesis...
...Sueoka grew the algae in a nutrient medium containing an isotope of nitrogen with an extra neutron in its nucleus. Since DNA contains nitrogen, newly synthesized cells become "labeled" or marked with the heavy nitrogen...
After allowing them to divide in the medium for several days, Sueoka transferred the cells with heavy DNA to a medium with normal nitrogen. Two hours later he extracted some DNA from these cells and found that it had a molecular density half-way between heavy and normal DNA. It appeared as though the heavy DNA had separated into two heavy strands and each had manufactured a new partner containing normal nitrogen...